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Cell surface biotinylation studies showed that both GLYX-13 and ketamine led to increases in both NR2B and Glu R1 protein levels, as measured by Western analysis, whereas no changes were seen in m RNA expression (microarray and q RT-PCR).
A better understanding of the similarities and differences between NREM sleep and dexmedetomidine-induced sedation is essential for efforts to clarify the relationship between these two states.
This study tested the hypothesis that dexmedetomidine-induced sedation is homologous to sleep.
GLYX-13, unlike ketamine, produced its antidepressant-like effect when injected directly into the medial prefrontal cortex (MPFC).
These results suggest that GLYX-13 produces an antidepressant-like effect without the side effects seen with ketamine at least in part by directly modulating NR2B-containing NMDARs in the MPFC.